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Community Reviews. Showing Rating details. More filters. Sort order. Kurzgeschichten von Knut Hamsum zum Teil unter Pseudonym in Tageszeitungen erschienen sind hier erstmals zusammengetragen worden und bilden ein grosses Ganzes. While a fascinating look at an author in the process of developing his own style, it's Larsen's comments which are the real treat here, subtly showing how Hamsun tried to rework the short stories post-publication and in doing so stripped them of their original qualities.
Luckily, all the stories are presented in their original form here. Libri di strada rated it really liked it Apr 27, Robert rated it it was amazing Jul 03, Elemesztakaosz rated it liked it Jan 15, Lasse rated it really liked it May 31, Josephine rated it liked it Apr 10, Paraph added it Dec 02, Hilja by Cucina Povera. Using the spellbinding effects of chants, minimalist synths, and repetition, Maria Rossi paints a picture of strange and fraught beauty.
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L'Offrande Narayan Nava Segah Devi Sthothra Sabat Sabing - Les Consonnes I-XIII Materials and Methods: Using experimental tumors generated in nude mice, we set up a technique for short-term culture of HCC fragments. We applied this technique to six human HCC samples obtained from surgical resection.
Results: HCC fragments in culture retain their morphology and viability for at least 48 h, permitting an in vitro analysis of the effect of sorafenib on the Extracellular signal-regulated kinase ERK cascade. HCC exhibit heterogeneous individual responses, ranging from potent inhibition to paradoxical activation of this oncogenic cascade. Conclusion: Our observations highlight the heterogeneous sensitivity of HCC to sorafenib, and point to the potential interest of short-term culture of tumor fragments for personalizing the medical treatment of HCC.
Hepatocellular carcinoma HCC is the most frequent form of primary liver cancer 1. Sorafenib, a kinase inhibitor that is active against RAF kinases, is currently the only medical treatment proved to extend the survival of patients with advanced HCC 2. Sorafenib essentially acts as a stabilizer of tumor growth, a fact that complicates the individual evaluation of its efficacy 2 , 3. Consequently, little is known about the heterogeneity of individual responses of patients to sorafenib. We recently found great differences among a panel of HCC cell lines, some being more resistant to sorafenib than others 4.
We examined the rational basis for this heterogeneity, and observed that the antiproliferative efficacy of sorafenib correlates with its ability to control the ERK kinase cascade in each cell line. In resistant HCC cells, sorafenib lost its ability to inhibit, and sometimes even paradoxically activated the ERK kinase cascade 4.
Fragments de Sud (French Edition) - Kindle edition by Florence Clerfeuille. Download it once and read it on your Kindle device, PC, phones or tablets. Qu'ils vivent au cœur de l'Amazonie brésilienne, sous la pluie de l'île chilienne de Chiloé, sur l'Altiplano bolivien ou dans le Sahara algérien, les héros de ces.
These observations point to the importance of the ERK kinase cascade as a therapeutic target of sorafenib in HCC, and indicate that HCC cells may not all be equally sensitive to sorafenib 4. In the present work, our aim was to translate these observations to a more clinically-relevant setting. Using experimental tumors grown as xenografts and clinical samples obtained from surgical resections, we validated a technique for the short-term culture of tumour fragments and used it to examine the sensitivity of individual HCC to sorafenib.
Cell lines and cultures. Hep3B cells were obtained from Dr.
Hepatocytic differentiation was verified during the course of the experiments by measuring alpha-fetoprotein secretion in the culture medium data not shown. Baseline characteristics of the patients with hepatocellular carcinoma. Research carried out on animals at our University complies with French standards of ethics, and was performed after approval by the local Ethics Committee CREMEA, approval number: Mice were killed by cervical dislocation prior to tumor recovery.
Patients' consent was obtained for the use of resected tumors. A summary of the pathological findings and baseline characteristics of each patient is given in Table I. Antibodies and chemical reagents. Immunoblot analysis. Immunoblots were performed with relevant antibodies and the Enhanced chemiluminescence reaction was used for revelation.
Lactate dehydrogenase LDH release assay. All analyses were performed on the culture medium collected at different time points. Statistical analyses.